DNA

Part:BBa_K2715021:Design

Designed by: Daniel Partridge   Group: iGEM18_Nottingham   (2018-10-08)


C.difficile Toxin A promoter 5' UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The translation initiation site was predicted using the BPROM algorithm (see references below), and the 5' UTR region identified.

Source

This basic part is the 5' UTR portion of the tcdA toxin gene regulatory region from the Gram-positive organism Clostridium difficile.


References

V. Solovyev, A Salamov (2011) Automatic Annotation of Microbial Genomes and Metagenomic Sequences. In Metagenomics and its Applications in Agriculture, Biomedicine and Environmental Studies (Ed. R.W. Li), Nova Science Publishers, p. 61-78